Coding
Endolysin

Part:BBa_K5311006

Designed by: Carlos Perez de Arenaza, Jose Luis Perez and Roger Planell   Group: iGEM24_UPF-Barcelona   (2024-09-24)
Revision as of 09:04, 29 September 2024 by Carlospdag (Talk | contribs) (Biology)

CAP 10-3 Endolysin

The CAP 10-3 endolysin is an enzyme derived from a bacteriophage that specifically targets and lyses Cutibacterium acnes. Its inclusion in our project is essential, as it allows us to regulate the growth of Cutibacterium acnes, ensuring a balanced interaction between the patient’s original skin microbiota and the newly introduced microbiota from our product, thereby preventing dysbiosis.

The primary function of this enzyme is to induce cell death in the transformed Cutibacterium acnes. It achieves this by degrading the peptidoglycan links in the bacterial cell wall, creating pores that destabilize the cell membrane. Ultimately, the exposed Cutibacterium acnes cells succumb to osmotic pressure, leading to their death.[1]

Sequencing

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 266
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 34
    Illegal NgoMIV site found at 445
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 193

Biology

The CAP 10-3 endolysin is an enzyme derived from a bacteriophage that specifically targets and lyses Cutibacterium acnes. Its inclusion in our project is essential, as it allows us to regulate the growth of Cutibacterium acnes, ensuring a balanced interaction between the patient’s original skin microbiota and the newly introduced microbiota from our product, thereby preventing dysbiosis.

The primary function of this enzyme is to induce cell death in the transformed Cutibacterium acnes. It achieves this by degrading the peptidoglycan links in the bacterial cell wall, creating pores that destabilize the cell membrane. Ultimately, the exposed Cutibacterium acnes cells succumb to osmotic pressure, leading to their death.[1]

[edit]
Categories
Parameters
chassisCutibacterium acnes