Coding

Part:BBa_K5271003:Design

Designed by: Yin Yan Chan   Group: iGEM24_HKPOLYU   (2024-09-28)
Revision as of 09:00, 29 September 2024 by Carriechan1 (Talk | contribs) (→‎Design Notes)


HER2-binding peptide -scrambled


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Our preliminary results when it is joined with the HER2 nanobody by a linker, the linker should avoid cysteine residues since it potentially reduces the solubility of the dual targeting nanobody in prokaryotic expression system.

Source

The HER2 specific nanobody is an antigen-binding fragments that are derived from Camelus dromedarius heavy-chain antibodies and have advantageous characteristics compared with mAbs and their derived fragments for in vivo targeting [Hamers-Casterman et al., 1993] The EGFR specific nanobody was isolated from a phage library generated from Llama glama lymphocytes that had been immunized with A431 epidermoid carcinoma cells [Roovers et al., 2007]

References