Part:BBa_K5311006
CAP 10-3 Endolysin
The CAP 10-3 endolysin is an enzyme derived from a bacteriophage that specifically targets and lyses Cutibacterium acnes. Its inclusion in our project is essential, as it allows us to regulate the growth of Cutibacterium acnes, ensuring a balanced interaction between the patient’s original skin microbiota and the newly introduced microbiota from our product, thereby preventing dysbiosis.
The primary function of this enzyme is to induce cell death in the transformed Cutibacterium acnes. It achieves this by degrading the peptidoglycan links in the bacterial cell wall, creating pores that destabilize the cell membrane. Ultimately, the exposed Cutibacterium acnes cells succumb to osmotic pressure, leading to their death.[1]
Sequencing
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 266
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 34
Illegal NgoMIV site found at 445 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 193
Biology
| chassis | Cutibacterium acnes |