Part:BBa_K180014

Rock-paper-scissors - Paper primary plasmid

Composite part, consisting of BBa_K180005 and BBa_K180015, but with BBa_K180005 reading along the reverse strand, and BBa_K180015 reading along the forward strand.

BBa_K180005 reads along reverse strand, and BBa_K180015 reads along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180005) has no effect over GFP or RhlI expression in BBa_K180015.

GFP and RhlI are generated under tac pR regulation, whilst LuxR is produced constitutively.

Forms the 'paper' primary plasmid in Team:Southampton's rock-paper-scissors project design.

Usage and Biology

This part has been specified as a basic BioBrick, due to some of the component parts reading in reverse (unable to specify using the component BioBrick format).

To function as the 'Paper' in the rock-paper-scissors design by Team:Southampton, the part has to be used in conjunction with K180016, and pairs K180010 & K180013, K180017 & K180020.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 945
  Illegal NheI site found at 968
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 3104