Coding

Part:BBa_K5034206:Design

Designed by: Zongyu Guo   Group: iGEM24_Nanjing-China   (2024-09-11)
Revision as of 07:09, 29 September 2024 by Ji-yf (Talk | contribs) (→‎Design Notes)


Poly P -> NADP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to improve the efficiency of energy production and phosphorus accumulation of Shewanella, this element of E. coli was migrated to Shewanella. We believe that the introduction of NADP can promote electron transport in Shewanella. This part is obtained by using a chemical synthesis method, with the aim of expressing the protein in Shewanella and regulating cellular phosphorus metabolism and electron transfer.

Source

Inorganic polyphosphate/ATP-NAD kinase(PPNK) from Mycobacterium tuberculosis H37Rv. NCBI reference sequence: NC_000962.3:1918746-1919669

References

Itoh, H., & Shiba, T. (2004). Polyphosphate synthetic activity of polyphosphate:AMP phosphotransferase in Acinetobacter johnsonii 210A. Journal of Bacteriology, 186(15), 5178-5181. doi:10.1128/jb.186.15.5178-5181.2004