Primer

Part:BBa_K5269028

Designed by: Yuichiro Nakai   Group: iGEM24_TokyoTech   (2024-09-28)
Revision as of 06:40, 29 September 2024 by Nakaiyuichiro (Talk | contribs)

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pBluescript II SK(-) In-Fusion reverse primer 2

This reverse primer is used with pBluescript II SK(-) In-Fusion forward primer 2 (BBa_K5269029) to linearize the plasmid. This allows insertion of T7 promoter, T7 terminator and shRNA with Golden Gate Assembly Site (BBa_K5269025).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None