Part:BBa_K5526000
Azurin
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
New Basic Part: BBa_K5526000 (Azurin)
Profile
Name: Azurin
Base Pairs: 444bp
Origin: Pseudomonas aeruginosa, synthesized.
Properties: Azurin is a cupredoxin protein isolated from Pseudomonas aeruginosa that can enter cancer cells and induce apoptosis.
Usage and Biology
Azurin is a cupredoxin protein isolated from Pseudomonas aeruginosa that can enter cancer cells and induce apoptosis. It interacts with specific molecules on the surface of cancer cells, disrupting intracellular signalling pathways. Azurin is primarily in the stages of basic research and preclinical trials and has not yet become a standard clinical treatment.
Cultivation, Purification, and SDS-PAGE
Azurin is a gene that encodes a chemical drug, which can release substances to kill tumor cells. We applied PCR on the genes Azurin (444bp) and pUC57-plldr (3150bp). Agarose gel electrophoresis was used to check the length of our PCR production to ensure success. The result shows that the pUC57-plldr (plactate 1) has a length of 3150bp, and the Azurin has a length of 444bp.
After the optimal protein expression conditions were obtained, the constructed strains were expanded for culture. 5 mM lactic acid was used to induce the actual expression level of tumor drugs in EcN1917. The experimental results are shown in Figure 3. The size of the Azurin protein is about 19kDa. A large amount of target protein indicates that the Azurin protein is successfully expressed. In the future, we will further study the expression and tumor inhibition effect of the constructed probiotics in the tumor environment.
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