Coding

Part:BBa_K5291028:Design

Designed by: Haosheng Lin   Group: iGEM24_BNUZH-China   (2024-09-25)
Revision as of 05:36, 29 September 2024 by Kendy (Talk | contribs) (→‎References)

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nadM: bifunctional nicotinamide-nucleotide adenylyltransferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use it primarily to consider the role of nadM in the de novo and salvage pathways of NAD+ biosynthesis. NadM encoded by nadM catalyze the adenylylation of the nicotinic acid mononucleotide precursor to nicotinic acid dinucleotide followed by its amidation to NAD+. Given the properties of this enzyme, we decided to use NadM to increase the total amount of NAD+ within the engineered bacteria.

Source

Francisella tularensis strain Schu S4

References

Sorci L, Martynowski D, Rodionov DA, Eyobo Y, Zogaj X, Klose KE, Nikolaev EV, Magni G, Zhang H, Osterman AL. Nicotinamide mononucleotide synthetase is the key enzyme for an alternative route of NAD biosynthesis in Francisella tularensis. Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3083-8. doi: 10.1073/pnas.0811718106. Epub 2009 Feb 9. PMID: 19204287; PMCID: PMC2651253.