Coding

Part:BBa_K5291028:Design

Designed by: Haosheng Lin   Group: iGEM24_BNUZH-China   (2024-09-25)
Revision as of 05:35, 29 September 2024 by Kendy (Talk | contribs) (→‎Design Notes)


nadM: bifunctional nicotinamide-nucleotide adenylyltransferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use it primarily to consider the role of nadM in the de novo and salvage pathways of NAD+ biosynthesis. NadM encoded by nadM catalyze the adenylylation of the nicotinic acid mononucleotide precursor to nicotinic acid dinucleotide followed by its amidation to NAD+. Given the properties of this enzyme, we decided to use NadM to increase the total amount of NAD+ within the engineered bacteria.

Source

Francisella tularensis strain Schu S4

References