Coding

Part:BBa_K5291026:Design

Designed by: Haosheng Lin   Group: iGEM24_BNUZH-China   (2024-09-25)
Revision as of 04:45, 29 September 2024 by Kendy (Talk | contribs) (→‎References)

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pntB: β subunit of membrane-bound proton-translocating pyridine nucleotide transhydrogen


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Considerations in the design of pntB include their important role in catalytic hydrogen transfer between NADH and NADPH. Considering that the overexpression of BcsAB by engineered bacteria to synthesize bacterial cellulose requires a considerable amount of NADPH, we decided to introduce PntAB to increase the intracellular level of NADPH. PntAB, which is constructed of α and β subunits and is encoded by pntA and pntB, can enhance the intracellular availability of NADPH when expressed.

Source

Escherichia coli str. K-12 substr. MG1655

References

1.Clarke DM, Loo TW, Gillam S, Bragg PD. Nucleotide sequence of the pntA and pntB genes encoding the pyridine nucleotide transhydrogenase of Escherichia coli. Eur J Biochem. 1986 Aug 1;158(3):647-53. doi: 10.1111/j.1432-1033.1986.tb09802.x. PMID: 3525165.
2.Shi A, Zhu X, Lu J, Zhang X, Ma Y. Activating transhydrogenase and NAD kinase in combination for improving isobutanol production. Metab Eng. 2013 Mar;16:1-10. doi: 10.1016/j.ymben.2012.11.008. Epub 2012 Dec 14. PMID: 23246519.
3.Spielmann A, Baumgart M, Bott M. NADPH-related processes studied with a SoxR-based biosensor in Escherichia coli. Microbiologyopen. 2019 Jul;8(7):e00785. doi: 10.1002/mbo3.785. Epub 2018 Dec 25. PMID: 30585443; PMCID: PMC6612552.