Composite

Part:BBa_K5115040

Designed by: Liyue Chen   Group: iGEM24_Fudan   (2024-09-19)
Revision as of 03:45, 29 September 2024 by Chenliyue (Talk | contribs)


ribozyme connected: RcnR_C35L, MTA, Hpn

contributed by Fudan iGEM 2023

Introduction

This composite part combines BBa_K5115033(ribozyme+RBS+RcnR_C35L+stem-loop), BBa_K5115035(ribozyme+RBS+MTA+stem-loop) and BBa_K5115036(ribozyme+RBS+hpn+stem-loop) in our ribozyme-assisted polycistronic co-expression system:pRAP. To get more information about pRAP, please check part wiki of 2022 Fudan iGEM .

MTA is a protein that can bind with nickel ions to reduce its toxity to the 'E.coli'. The hpn is a protein that can sequester metals that accumulate internally to reduce nickel's toxity to the 'E.coli'. RcnR_C35L can regulate the nickel ion channel proteins in the cell membrane to tune the nickel ion transport rate.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 303
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 582
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[edit]
Categories
Parameters
None