Part:BBa_K5115063

hox and hyp, with EP targeted hoxF

Introduction

This composite part combines all the subunit of the hydrogenase in our ribozyme-assisted polycistronic co-expression system：pRAP. It's worth mention that, in this part, the subunit hoxF is fused with EP, check BBa_K51150058(hoxF fused with EP)for more details. To learn more about the hydrogenase, please check BBa_K51150020(hox and hyp operon). To get more information about pRAP, please check part wiki of 2022 Fudan iGEM .

Usage and biology

This part is crucial in BBa_K51150067(mineral, F module), click on the link to view the experiment details.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 485
  Illegal BglII site found at 563
  Illegal BglII site found at 9209
  Illegal BglII site found at 10027
  Illegal BglII site found at 10320
  Illegal BamHI site found at 956
  Illegal XhoI site found at 493
  Illegal XhoI site found at 685
  Illegal XhoI site found at 1163
  Illegal XhoI site found at 3402
  Illegal XhoI site found at 5231
  Illegal XhoI site found at 6393
  Illegal XhoI site found at 8232
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1256
  Illegal NgoMIV site found at 1936
  Illegal NgoMIV site found at 4543
  Illegal NgoMIV site found at 4650
  Illegal NgoMIV site found at 4920
  Illegal NgoMIV site found at 5766
  Illegal NgoMIV site found at 5998
  Illegal AgeI site found at 446
  Illegal AgeI site found at 5718
  Illegal AgeI site found at 11103
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 267
  Illegal BsaI site found at 429
  Illegal BsaI site found at 3056
  Illegal BsaI.rc site found at 9257
  Illegal BsaI.rc site found at 9761
  Illegal SapI.rc site found at 378