Coding

Part:BBa_K209442

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-03)
Revision as of 21:04, 21 October 2009 by Brhau (Talk | contribs)

AarI B-C part, M3/M2 chimera

M3/M2 chimera DREADD flanked by AarI BC sites.

Usage and Biology

This M3/M2 chimeric receptor consists of the human M3 muscarinic receptor with the i3 loop from M2 muscarinic receptor. This is an engineered version of the GPCR that can be activated by the otherwise inert small molecule, clozapine-N-oxide.

Reference: Delineation of muscarinic receptor domains conferring selectivity of coupling to guanine nucleotide-binding proteins and second messengers. Wess J, Bonner TI, Dörje F, Brann MR. Mol Pharmacol. 1990 Oct;38(4):517-23.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1314
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 936
    Illegal XhoI site found at 128
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None