Intermediate

Part:BBa_K218006:Design

Designed by: Jeremy Kubik   Group: iGEM09_Calgary   (2009-10-19)
Revision as of 21:01, 21 October 2009 by Jfkubik (Talk | contribs) (→‎Design Notes)

LuxPQ followed by terminator and LuxOU generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2209
    Illegal BglII site found at 2427
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3509
    Illegal BsaI.rc site found at 4886
    Illegal SapI.rc site found at 2177
    Illegal SapI.rc site found at 4848


Design Notes

The LuxPQ and LuxOU operons each have an internal RBS and terminator. Additional terminators were cloned downstream of each operon to ensure insulation.

Source

LuxPQ - GenBank Accession: NC_009784 Vibrio harveyi ATCC BAA-1116 chromosome II, REGION: 579654..580751.

LuxOU - GenBank Accession# L26221 LuxO Protein ID# AAD12736.1 LuxU Protein ID# AAD12737.2

B0015 - Registry of Standard Biological Parts

R0040 - Registry of Standard Biological Parts

References