Part:BBa_K5115059

hoxU fused with EP

Introduction

Combine hoxU directly with EP. The hoxU is a subunit of the hydrogenase, check it in BBa_K5115020(hox and hyp operon). EP can direct external proteins into carboxysomes, check it in BBa_K51150002(EP).

Usage and Biology

Binding hoxU with EP can direct the hydrogenase into the carboxysome, creating a safe and stable environment for the enzyme to work.

Characterization

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 410
  Illegal BglII site found at 488
  Illegal XhoI site found at 418
  Illegal XhoI site found at 610
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 371
  Illegal AgeI site found at 1370
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 192
  Illegal BsaI site found at 354
  Illegal SapI.rc site found at 303