Composite

Part:BBa_K5115058

Designed by: Yi Shi   Group: iGEM24_Fudan   (2024-09-19)
Revision as of 01:44, 29 September 2024 by Chenliyue (Talk | contribs)

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hoxF fused with EP

contributed by Fudan iGEM 2023

Introduction

Combine hoxF directly with EP. The hoxF is a subunit of the hydrogenase, check it in BBa_K5115020(hox and hyp operon). EP can direct external proteins into carboxysomes, check it in BBa_K51150002(EP).

Usage and Biology

Binding hoxF with EP can direct the hydrogenase into the carboxysome, creating a safe and stable environment for the enzyme to work.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 577
    Illegal BglII site found at 1395
    Illegal BglII site found at 1688
    Illegal XhoI site found at 22
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 456
    Illegal NgoMIV site found at 994
    Illegal NgoMIV site found at 1204
    Illegal NgoMIV site found at 1516
    Illegal AgeI site found at 2471
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 625
    Illegal BsaI.rc site found at 1129


References

[edit]
Categories
Parameters
None