Coding

Part:BBa_K209402:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-02)
Revision as of 20:59, 21 October 2009 by Brhau (Talk | contribs) (→‎Source)

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AarI A-B part, signal sequence-FLAG-YFP [SSFYFP]


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 346
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 346
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 128
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 346
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 346
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 84


Design Notes

Cloned into pDONR221.

Source

Signal sequence obtained from Bruce Conklin lab, Gladstone.

References