Part:BBa_K5241004
Mel
The ADE2 gene is combined with the yeast gal1 promoter, the enhanced green fluorescent protein gene egfp, and the CYC1 terminator.
genetic map：
Source:Pseudomonas maltophilia
Description:
The role of the mel gene in brewing yeast is mainly as a reporter gene. The tyrosinase enzyme it encodes can catalyze the conversion of tyrosine to dopamine, which is then further converted into melanin. This produced melanin is non-toxic and harmless, allowing for the direct observation of a black phenotype on the culture medium without the need to add color-developing compounds or use special instruments.
Result
We successfully expressed the mel gene,the melanin expressed by the mel gene in brewing yeast appears red, possibly because a larger amount of pheomelanin is produced.
Reference documentation
[1] Ruohola, H., Liljeström, P. L., Torkkeli, T., Kopu, H., Lehtinen, P., Kalkkinen, N., & Korhola, M. (1986). Expression and regulation of the yeast MEL1 gene. FEMS Microbiology Letters, 34(2), 179–185.
[2] Naumov, G., Naumova, E., Turakainen, H., Suominen, P., & Korhola, M. (1990). A new family of polymorphic genes in Saccharomyces cerevisiae: alpha-galactosidase genes MEL1-MEL7. Molecular Genetics and Genomics, 224(1), 119–128.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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