Part:BBa_K316012

TEV protease S219P autocatalysis resistant variant

TEV protease S219P autocatalysis resistant variant. This part had been reversed for the 3' strand in order to reduce any read-through that may be caused by upstream elements.

Introduction :

This is the nuclear inclusion protease, endogenous to Tobacco Etch Virus and is used in the late lifecycle to cleave polyprotein precursors. The recognition sequence is ENLYFQG/S 1 between QG or QSDue to it’s stringent sequence specificity, TEV is commonly used to cleave genetically engineered proteins.

Uses:

TEV proteinase is used to cleave fusion proteins. It is useful due to its high degree of specificity1 and potential to be used in vivo or in vitro applications.

Auto-inactivation:

Wild type TEV protease also cleaves itself at Met 218 and Ser 2192. This leads to auto-inactivation of the TEV protease and progressive loss of activity of the protein. The rate of inactivation is proportional to the concentration of protease. More stable Mutants have been produced by single amino acid substitutions S219V (AGC(serine) to GTG(valine) and S219P (AGC(serine) to CCG(proline)3.

Table I.

Kinetic parameters for wild-type and mutant TEV proteases with the peptide substrate TENLYFQSGTRR-NH2. From original paper by Kapust et.al. 20013

Enzyme	Km (mM)	kcat (s-1)	kcat /Km (mM-1 s-1)
Wild type	0.061 ± 0.010	0.16 ± 0.01	2.62 ± 0.46
S219V	0.041± 0.010	0.19 ± 0.01	4.63 ± 1.16
S219P	0.066 ± 0.008	0.09 ±0.01	1.36 ± 0.22

S219V* - retains same activity as wild type

S219P* - virtually imperivious to autocatalysis

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 32
  
• 1000
  COMPATIBLE WITH RFC[1000]

References

<biblio>

1. 1 pmid=8179197
2. 2 pmid=7793070
3. 3 pmid=2047602

</biblio>

For more information about our project please visit our [http://2010.igem.org/Team:Imperial_College_London wiki].

Contribution

• Group: TecCEM, iGEM 2024
• Authors: Giovana Andrea Osorio León, Ana Laura Torres Huerta, Aurora Antonio Pérez, Lorena Gallegos Solís
• Summary: The sequences of TEV proteases S219P and 1LVM were compared to determine if TEV S219P exhibits greater efficiency and interaction with a substrate. Sequence alignment was performed using ESPript3, and both enzymes were modeled with AlphaFold for structural analysis. Additionally, heatmaps of protein stability were generated using Protein-Sol and molecular docking was carried out with HADDOCK 2.4 and BIOVIA Discovery Studio Visualizer to compare their interactions.

Documentation

File:Bba-k316012-3d-model-1.png

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