Part:BBa_K5490006

mNFAT

murine NFAT regulatory domain In its natural state, NFAT contains two Nuclear Localization Signals (NLS) that enable nuclear translocation. However, in this engineered version, one NLS has been removed, leaving only a single NLS to reduce background transcription. The NFAT domain is activated through phosphorylation by calcineurin in response to calcium signaling. This domain is critical for calcium-dependent activation in cellular processes, as the increase in cytosolic calcium activates calmodulin, which, in turn, activates calcineurin.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1116
  Illegal PstI site found at 934
  Illegal PstI site found at 1188
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1116
  Illegal NheI site found at 505
  Illegal PstI site found at 934
  Illegal PstI site found at 1188
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1116
  Illegal BamHI site found at 983
  Illegal XhoI site found at 1222
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1116
  Illegal PstI site found at 934
  Illegal PstI site found at 1188
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1116
  Illegal PstI site found at 934
  Illegal PstI site found at 1188
  Illegal NgoMIV site found at 388
  Illegal NgoMIV site found at 408
  Illegal NgoMIV site found at 424
  Illegal NgoMIV site found at 889
  Illegal NgoMIV site found at 1173
  
• 1000
  COMPATIBLE WITH RFC[1000]

Meško M, Lebar T, Dekleva P, Jerala R, Benčina M. Engineering and Rewiring of a Calcium-Dependent Signaling Pathway. ACS Synth Biol. 2020 Aug 21;9(8):2055-2065. doi: 10.1021/acssynbio.0c00133. Epub 2020 Jul 20. PMID: 32643923; PMCID: PMC7467823.