Reporter
REPORTER W

Part:BBa_K5490024:Design

Designed by: IOANNIS VASILEIOS ELAFROPOULOS   Group: iGEM24_IOANNINA   (2024-09-25)
Revision as of 23:56, 28 September 2024 by Tzonissss13 (Talk | contribs) (→‎Design Notes)

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REPORTER FOR TESTING WNV GRNA TARGET SITES


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3271
    Illegal XbaI site found at 3493
    Illegal PstI site found at 1490
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3271
    Illegal PstI site found at 1490
    Illegal NotI site found at 3476
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3271
    Illegal BamHI site found at 3484
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3271
    Illegal XbaI site found at 3493
    Illegal PstI site found at 1490
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3271
    Illegal XbaI site found at 3493
    Illegal PstI site found at 1490
    Illegal NgoMIV site found at 1652
    Illegal NgoMIV site found at 3029
    Illegal NgoMIV site found at 3050
    Illegal NgoMIV site found at 3365
    Illegal AgeI site found at 2744
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We ordered an insert containing a degradation signal derived from the pcDNA3.3_d2eGFP construct, a T2A peptide, and the luciferase gene with three target sequences. To facilitate directional cloning, we included two restriction sites: HindIII upstream and BamHI downstream. These allowed us to clone the insert into a plasmid already available in the lab. The plasmid, pEGFPC1, contains a CMV promoter, an EGFP gene, and a multicloning site that includes both the HindIII and BamHI restriction sites, with a poly-A tail downstream.

Source

DISIGNED BY IGEM IOANNINA 2024

References