Translational_Unit
EGFP

Part:BBa_K5490022

Designed by: IOANNIS VASILEIOS ELAFROPOULOS   Group: iGEM24_IOANNINA   (2024-09-25)
Revision as of 23:46, 28 September 2024 by Tzonissss13 (Talk | contribs)

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EGFP

A basic part of the composite part BBa_K5490024, is an EGFP protein .Fused with a degradation signal ,the BBa_K5490024 significantly reduces its half-life in the cytosol, making it highly suitable for applications such as RNAi and Cas13 studies. This shortened half-life allows for dynamic assessment of gene silencing and real-time promoter strength analysis through live-cell imaging

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 806
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 806
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 806
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 806
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None