Composite

Part:BBa_K5416060

Designed by: Jixiao Wu   Group: iGEM24_Imperial-College   (2024-09-28)
Revision as of 21:55, 28 September 2024 by Registry (Talk | contribs)

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pT7LacO + P22 + HRT2-SP + T7 term

This is a composite part consisting of two CDS: P22 and HRT2-SP protein, placed downstream of pT7-LacO promoter for inducible overexpression. P22 (BBa_K3187021) is the bacteriophage P22 mature virion capsid protein fused with a His-tag. The HRT2-SP encodes a truncated cis-prentyltransferase HRT2(BBa_K5416000) derived from H. brasiliensis that is recombined with a scaffold-protein (SP, BBa_K3187017) domain that can interact with the inner surface of the P22 capsid. We here to report this designed composite part to be capable of forming a virus-like-particle (VLP) as an artificial organelle with a compartmentalized environment for rubber production.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1354
    Illegal AgeI site found at 507
    Illegal AgeI site found at 1755
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 625


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Categories
Parameters
None