Composite
Part:BBa_K5416060
Designed by: Jixiao Wu Group: iGEM24_Imperial-College (2024-09-28)
pT7LacO + P22 + HRT2-SP + T7 term
This is a composite part consisting of two CDS: P22 and HRT2-SP protein, placed downstream of pT7-LacO promoter for inducible overexpression. P22 (BBa_K3187021) is the bacteriophage P22 mature virion capsid protein fused with a His-tag. The HRT2-SP encodes a truncated cis-prentyltransferase HRT2(BBa_K5416000) derived from H. brasiliensis that is recombined with a scaffold-protein (SP, BBa_K3187017) domain that can interact with the inner surface of the P22 capsid. We here to report this designed composite part to be capable of forming a virus-like-particle (VLP) as an artificial organelle with a compartmentalized environment for rubber production.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1354
Illegal AgeI site found at 507
Illegal AgeI site found at 1755 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 625
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Categories
Parameters
None |