Part:BBa_K5477038

CYP3A4-pGAL1/10-POR detox module against a wide array of contaminants

In our system, the CYP3A4-pGAL1/10-POR composite part combines the CYP3A4 enzyme and cytochrome P450 oxidoreductase (POR) to form a highly efficient detoxification unit. The pGAL1/10 bidirectional promoter drives the expression of both CYP3A4 and POR simultaneously but in opposite directions. CYP3A4, a key phase I enzyme, is responsible for oxidizing around 50% of all clinically used drugs and environmental toxins. POR provides the electrons required for these oxidation reactions by transferring electrons from NADPH to CYP3A4, ensuring efficient metabolism of harmful xenobiotics and pharmaceuticals.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 1483
  Illegal PstI site found at 2486
  Illegal PstI site found at 2683
  Illegal PstI site found at 3067
  Illegal PstI site found at 3507
  Illegal PstI site found at 3567
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 2486
  Illegal PstI site found at 2683
  Illegal PstI site found at 3067
  Illegal PstI site found at 3507
  Illegal PstI site found at 3567
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 1483
  Illegal PstI site found at 2486
  Illegal PstI site found at 2683
  Illegal PstI site found at 3067
  Illegal PstI site found at 3507
  Illegal PstI site found at 3567
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 1483
  Illegal PstI site found at 2486
  Illegal PstI site found at 2683
  Illegal PstI site found at 3067
  Illegal PstI site found at 3507
  Illegal PstI site found at 3567
  Illegal NgoMIV site found at 2889
  Illegal NgoMIV site found at 3008
  Illegal AgeI site found at 1894
  
• 1000
  COMPATIBLE WITH RFC[1000]