Plasmid_Backbone
Part:BBa_K5109060:Design
Designed by: Lisa Faccincani Group: iGEM24_Uni-Padua-IT (2024-09-28)
Revision as of 20:19, 28 September 2024 by Lisafaccincani (Talk | contribs)
We designed primers forward and reverse overlapping the restriction site, both of them with a single mismatch on the second T of the site "CATATG".
Primer forward: 5’ – CCGCATAAGGTGCACTCTCAGTACAAT – 3’ Primer reverse: 5’ – GTGCACCTTATGCGGTGTGAAATACCG – 3’
After the mutagenesys, the restriction site was changed from CATATG to CATAAG.