Part:BBa_K5078002

PTC amiRNA

An aritificial micro RNA used to silence the expression of the Phosphate Transport Channel 1 (PTC1) gene in Chlamydomonas reinhardtii thus removing the PTC1 transport protein from the central vacuole of the cell and preventing leakage of phosphate back into the environment in which our modified C. reinhardtii cell is placed.

Figure 1. Ptc amiRNA in a level 0 plasmid for later golden gate assembly.

Assembly verification

Successful insertion of the Ptc amiRNA was confirmed by PCR of the colonies, showing an increase of 100 bp of the PCR product.

Figure 2. PCR verification of cloning of Ptc amiRNA.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 112
  Illegal PstI site found at 205
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 85
  Illegal PstI site found at 112
  Illegal PstI site found at 205
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 112
  Illegal PstI site found at 205
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 112
  Illegal PstI site found at 205
  
• 1000
  COMPATIBLE WITH RFC[1000]