Composite

Part:BBa_K243018

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-14)
Revision as of 20:42, 21 October 2009 by Sarah (Talk | contribs)

His-DigA-Split Linker-Fok_i

This part unites some of our parts and their features.


Usage and Biology

This composite part is one part of our universal endonuclease and it needs another composite part like BBa_K243024 to built to a functional heterodimer. The DigA tag leads the part to DNA which is hybridized with an Digoxigenin labeled oligonucleotide. The split linker makes a distance from 51bp between the DigA and the linked Fok_i protein domain. The His-Tag serve as purification tag for Ni-NTA column purification.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 272
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aHis-DigA-Split Linker-Fok_i