Part:BBa_K5078006
PL1 Transcriptional Unit for NosZ
pL1-NosZ-D.denit (pL1-D.den) is the combination of the Psad Promoter+5' UTR (BBa_K3002001), NosZ D. denitrificans the nitrous oxide reductase gene from Dechloromonas denitrificans codon optimized for expression in Chlamydomonas reinhardtii (BBa_K5078000), the N-terminator sequence of the fluorescent reporter gene mCherry (BBa_K4770013), and the Psad Terminator (BBa_k3002002). pL1-D.den is the nitrogen half of our completed nutrient uptake plasmid (BBa_K5078009). pL1-D.den is similar to our other nitrogen uptake plasmid pL1- NosZ-P.stuzeri (BBa_K5078005) in that they are both responsible for altering the nitrogen pathway of a host bacterium by forcing the host bacterium to undergo the final step of denitrification, the reduction of nitrous oxide (N₂O) into dinitrogen (N₂). By encoding for nitrous oxide reductase, which will act as the catalysis of a copper-dependent two electron reduction of N₂O into water and N₂ [1]. Additionally this has the benefit of preventing the greenhouse gas N₂O from entering the atmosphere and theoretically should cause the host bacterium to uptake more nitrogen from their environment. The difference between them is that pL1-D.den has a higher affinity for N₂O than pL1-NosZ-P-stuzeri [2]. We chose to use both,in different final level 2 plasmid builds, as we were unsure how they would function in Chlamydomonas reinhardtii.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 2941
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 2941
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 2941
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 2941
- 1000COMPATIBLE WITH RFC[1000]
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