Coding

Part:BBa_K209402

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-02)
Revision as of 20:34, 21 October 2009 by Brhau (Talk | contribs)

AarI A-B part, signal sequence-FLAG-YFP [SSFYFP]


Usage and Biology

Intended to be used as an N-terminal addition to G protein-coupled receptors. The signal sequence from prolactin is reported to increase surface expression of GPCRs and is cleaved off leaving the free FLAG epitope, which is recognized by the M1 antibody. The signal sequence is also necessary for the YFP (in this case Venus) to be translocated across the plasma membrane.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 346
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 346
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 128
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 346
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 346
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 84


[edit]
Categories
Parameters
n/aAarI A-B part, signal sequence-FLAG-YFP [SSFYFP]