Intermediate

Part:BBa_K218005:Design

Designed by: Jeremy Kubik   Group: iGEM09_Calgary   (2009-10-19)
Revision as of 20:34, 21 October 2009 by Jfkubik (Talk | contribs) (→‎References)

LuxOU generator with terminator upstream


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1053
    Illegal SapI.rc site found at 1015


Design Notes

No RBS was cloned into this intermediate because the LuxOU operons already has an internal RBS. Although there is an internal terminator as well, we have constructed the strong terminator B0015 after this coding sequence in order to provide further insulation of the circuit. The B0015 terminator was cloned upstream of the LuxOU generator because this intermediate will allow for subsequent cloning and insulation of LuxPQ upstream of this terminator.

Source

GenBank Accession# L26221 LuxO Protein ID# AAD12736.1 LuxU Protein ID# AAD12737.2

The source of the B0015 terminators and the R0040 promoter was the Registry of Standard Biological Parts.

References

Jeremy A. Freeman and Bonnie L. Bassler. Sequence and function of LuxU: a two-component phosphorelay protein that regulates quorum sensing in Vibrio harveyi. 1999. Journal of Bacteriology. Feb 1999, p. 899-906.