Coding

Part:BBa_K209400

Designed by: Benjamin Rhau   Group: iGEM09_UCSF   (2009-09-29)
Revision as of 20:25, 21 October 2009 by Brhau (Talk | contribs)

AarI B-C part, hM4D

hM4 DREADD flanked by AarI BC sites.

Usage and Biology

This is an engineered version of the M4 muscarinic receptor that can be activated by the otherwise inert small molecule, clozapine-N-oxide.

Reference: Evolving the lock to fit the key to create a family of G protein-coupled receptors potently activated by an inert ligand. Armbruster BN, Li X, Pausch MH, Herlitze S, Roth BL. Proc Natl Acad Sci U S A. 2007 Mar 20;104(12):5163-8. Epub 2007 Mar 2.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 184
    Illegal PstI site found at 979
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1215
    Illegal PstI site found at 184
    Illegal PstI site found at 979
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 184
    Illegal PstI site found at 979
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 184
    Illegal PstI site found at 979
    Illegal NgoMIV site found at 1086
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/membrane/receptor
Parameters
n/aAarI B-C part, hM4D