Coding

Part:BBa_K5097009

Designed by: Elber Lopez Hernandez, Kelly Acen, Runyararo Chaora   Group: iGEM24_Oneonta   (2024-09-27)
Revision as of 17:48, 28 September 2024 by Jazmine1274 (Talk | contribs)


SpeA (arginine decarboxylase)

Usage and Biology

This specific part codes for SpeA gene from E.coli. It expresses the enzyme arginine decarboxylase (Shaibe et al., 1985). This enzyme catalyzes the conversion of L-arginine into agmatine and carbon dioxide, the first step in the metabolic pathway that converts arginine into putrescine (Morris et al., 1969). The 2024 Oneonta iGEM designed this sequence for use in a base producing circuit. This part when co-expressed with SpeB (BBa_K0597010) will construct this pathway and produce putrescine from arginine.

File:Putrescine-pathway.jpg
Figure 1: Conversion of Arginine to Putrescine. Figure adapted from reference 3 (Charlier et al., 2019)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1389
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1167
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None