Composite

Part:BBa_K243014

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-14)
Revision as of 20:19, 21 October 2009 by Isabel (Talk | contribs)

His-FluA-Middle Linker-Fok_i

This part unites some of our parts. The combination of a Histidin-Tag, a Lipocalin-Tag and the linked protein domain Fok_a makes it possible to purify and detect the function of the protein in one step. The FluoresceinA tag allows the measurement by quenching and the coupling to an anticalin linked oligo.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 272
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/a His-FluA-Middle Linker-Fok_i