Coding

Part:BBa_K5233013:Design

Designed by: Tianshen TANG   Group: iGEM24_HUST-UEVE-UPSaclay   (2024-09-28)
Revision as of 16:49, 28 September 2024 by Registry (Talk | contribs)

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MHETase(PelB), a kind of tannase family protein that can degrade MHET


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1478
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1478
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1478
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1478
    Illegal NgoMIV site found at 54
    Illegal AgeI site found at 451
    Illegal AgeI site found at 1101
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

MHET is extracellular, and we need to use Amcut to secrete MHETase out of the cell to degrade MHET.


Source

It comes from A. mediterranei

References