Composite

Part:BBa_K5335023

Designed by: Ruichen Shen   Group: iGEM24_HZAU-China   (2024-09-28)
Revision as of 16:30, 28 September 2024 by Registry (Talk | contribs)

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A part (including a reporter gene) that induces and regulates downstream gene expression by salicyli

This part includes the LysR-type regulator NahR, the NahR binding sequence and the inducible promoter Psal, and a cyan fluorescent protein, amCyan. In this part, Amcyan was used as a reporter gene, and the expression level could be measured by light excitation at 453nm and detection at a wavelength of 486nm.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 77
    Illegal NgoMIV site found at 618
    Illegal AgeI site found at 1855
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None