Plasmid_Backbone

Part:BBa_K5143005:Design

Designed by: Jess Philippon   Group: iGEM24_UnivLyon1-INSALyon   (2024-07-29)
Revision as of 16:00, 28 September 2024 by Aymericperrel (Talk | contribs) (→‎Design Notes)

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backbone for integrating genetic elements into the Saccharomyces cerevisiae genome


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Illegal EcoRI site found at 3440
    Illegal EcoRI site found at 3967
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3440
    Illegal EcoRI site found at 3967
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3973
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3440
    Illegal EcoRI site found at 3967
    Illegal XhoI site found at 1605
    Illegal XhoI site found at 3446
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Illegal suffix found at 2
    Illegal EcoRI site found at 3440
    Illegal EcoRI site found at 3967
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3440
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI.rc site found at 714


Design Notes

We used BY4741 S. cerevisiae genomic sequences to design URA3' and URA5' homologous region and the URA3 selection marker in the backbone.

Source

test

References