Coding

Part:BBa_K5382136

Designed by: yuyanyan chen   Group: iGEM24_HUBU-4-CHN   (2024-09-26)
Revision as of 15:53, 28 September 2024 by Chenyuyanyan (Talk | contribs)


Cas9 handle-reverse repeating sequence in gRNA

The Cas9 handle ,namely stem-loop sequence of gRNA, refers to the reverse repeating sequence in its structure, which can be folded back to form a secondary structure, that is, a stem-loop structure, due to the hydrogen bond pairing between complementary bases. In gRNA, this conserved sequence helps stabilize gRNA binding to Cas9 protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None