Coding

Part:BBa_K5117025

Designed by: Jenny Sauermann, Lilli Kratzer, Katrin Lehmann   Group: iGEM24_TU-Dresden   (2024-09-28)
Revision as of 15:47, 28 September 2024 by Jesa98 (Talk | contribs)


BsEglS-L3

eglS gene of Bacillus subtilis, excluding its native signal peptide for secretion, encoding an endoglucanase (EC 3.2.1.4)

Downstream of the coding sequence, a rigid linker (L3) has been added encoding the amino acids GGGEAAAKGGG leading to the formation of an alpha helix.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 526
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None