Coding

Part:BBa_K5280129

Designed by: Hua XU   Group: iGEM24_HKUST-GZ   (2024-09-07)
Revision as of 14:33, 28 September 2024 by Hxu129 (Talk | contribs)


LexRO

LexRO is a recombinant protein which will dimerise in darkness and the dimers will bind to DNA, blocking the expression of downstream genes. When exposed to light, more specifically blue light (450~465nm), the dimers will deassiociate and lose the ability to repress gene expression. Therefore, the downstream genes will be expressed.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 205
    Illegal PstI site found at 445
    Illegal PstI site found at 514
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 205
    Illegal PstI site found at 445
    Illegal PstI site found at 514
    Illegal NotI site found at 587
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 205
    Illegal PstI site found at 445
    Illegal PstI site found at 514
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 205
    Illegal PstI site found at 445
    Illegal PstI site found at 514
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None