Composite

Part:BBa_K5453004

Designed by: Peipei Liu   Group: iGEM24_SZSAIS-Shenzhen   (2024-09-01)
Revision as of 13:30, 28 September 2024 by Peepl (Talk | contribs)


dcas9-sgRNA-pfkA-lac operator-lacI promoter-sgRNA-zwf-lac operator-lacI promoter

dcas9-sgRNA-pfkA-lac operator-lacI promoter-sgRNA1-zwf-lac operator-lacI promoter

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1096
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3375
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Subsequently, the plasmids R6k-dcas9-pfkA-zwf-M and pYB1c-Gatz-PGP-PGI were co-transformed into BW25113. Ninety-five colonies were randomly selected for fermentation experiments using glucose as the substrate, and their growth and production yields were screened.

[edit]
Categories
//function/dna
Parameters
function