Coding

Part:BBa_K5115054

Designed by: Yi Shi   Group: iGEM24_Fudan   (2024-09-19)
Revision as of 13:06, 28 September 2024 by Chenliyue (Talk | contribs)

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stayGold-GS

contributed by Fudan iGEM 2023

Introduction

The staygold is a green fluorescent protein (GFP) derived from the jellyfish Cytaeis uchidae. It has high brightness, high stability and low phototoxity,[1] which is a perfect choice for our design.

Usage and Biology

There actually exists a stayGold part in registry: BBa_K4491134. We add a Gly and a Ser to it as a linker, allowing it to link with EP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 638
    Illegal BsaI.rc site found at 658


References

  1. ↑ Hirano, M., Ando, R., Shimozono, S., Sugiyama, M., Takeda, N., Kurokawa, H., Deguchi, R., Endo, K., Haga, K., Takai-Todaka, R., Inaura, S., Matsumura, Y., Hama, H., Okada, Y., Fujiwara, T., Morimoto, T., Katayama, K., & Miyawaki, A. (2022). A highly photostable and bright green fluorescent protein. Nature Biotechnology, 40(7), 1132–1142.
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