Coding

Part:BBa_K5115070

Designed by: Yi Shi   Group: iGEM24_Fudan   (2024-09-19)
Revision as of 12:39, 28 September 2024 by Chenliyue (Talk | contribs)


YejM contributed by Fudan iGEM 2023

Introduction

YejM is an anti-adaptor protein that can regulate the synthesis of the LPS. In E.coli, LPS levels are under feedback control, achieved by FtsH-mediated degradation of LpxC. FtsH is a membrane- bound AAA+ protease, and its protease activity toward LpxC is regulated by essential membrane proteins LapB and YejM. YejM can compete with FtsH for LapB to inhibit LpxC degradation, thus guaranteed the expression level of LPS.[1]

Usage and Biology

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1440
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. ↑ Shu, S., & Mi, W. (2022). Regulatory mechanisms of lipopolysaccharide synthesis in Escherichia coli. Nature Communications, 13(1), 4576.
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