Regulatory

Part:BBa_K175046:Design

Designed by: Daniel Solis Escalante   Group: iGEM09_TUDelft   (2009-10-12)
Revision as of 19:58, 21 October 2009 by Danielsoes (Talk | contribs) (→‎References)

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Plasmid 1 for Transcriptional Cascade subproject TU Delft iGEM 09


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1436
    Illegal AgeI site found at 1548
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed to express RFP when it is placed inside E. coli. If this part is transformed together with BBa_K175047 the result is a delay in the expression of GFP after IPTG induction.

Source

This part was constructed using biobricks from the registry

References

This part was [http://2009.igem.org/Team:TUDelft/Synthetic_Transcriptional_Cascade:_The_plan designed] by the TUDelft 09 team. The design was based on the following works.

Hooshangi S, Thiberge S, Weiss R, 2005, [http://www.pnas.org/content/102/10/3581.abstract Ultrasensitivity and noise propagation in a synthetic transcriptional cascade], PNAS, 102,10 :3581–3586.

Rosenfeld N and Alon U, [http://www.ncbi.nlm.nih.gov/pubmed/12787666 Response Delays and Structure of Transcription Networks], J. Mol. Biol (2003) 329, 645-654.