Plasmid
Part:BBa_K5392015
Designed by: ZHENQIANG SUN Group: iGEM24_LIUAN-Nanjing (2024-09-24)
Vector-ZaTdT-KanR
Description
We incorporated the sequences of the ZaTdT gene into the pET28a vector. Then the vector plasmid was transfected into E.coli DH5-alpha competent cells for purification and amplification. In this way, plasmids with the ZaTdT gene were obtained.
Experiment
SDS-PAGE of ZaTdT
We transfected the ZaTdT gene-bonded pET28a plasmid into E.coli BL21(DE3) competent cell. After overnight, Colonies were then picked and performs protein expression. We identified the ZaTdT expression by SDS-PAGE and tested the incorporation of modified nucleotides by purified ZaTdT with polyacrylamide gel electrophoresis.
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