Plasmid
Part:BBa_K5143027:Experience
Designed by: Perrine Ferdinand Group: iGEM24_UnivLyon1-INSALyon (2024-09-27)
Revision as of 09:53, 28 September 2024 by Aymericperrel (Talk | contribs)
Applications of BBa_K5143027
The Venus/Ruby plasmid has been used in our project to test the activation of the GAP promoter and the ADH1 promoter in the BY4741 S. cerevisiae strain by measuring the fluorescence.
This plasmid has been digested by the XhoI restriction enzyme in order to release the Venus/Ruby fragment. Then, the Venus/Ruby fragment has been transformed in the BY4741 S. cerevisiae strain, and thanks to URA3_5' and URA3_3'homologous regions, this fragment has been inserted in the yeast genome by recombination.
Moreover, the fluorescence of this new strain has been measured in order to test the activation of the GAP promoter and the ADH1 promoter in the construct.