Coding
Part:BBa_K5276003:Design
Designed by: Zitao Qiu Group: iGEM24_UCAS-China (2024-09-26)
PhiC31 recombinase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1665
Illegal SapI.rc site found at 1356
Illegal SapI.rc site found at 1414
Illegal SapI.rc site found at 1519
Design Notes
Source
References
Roquet, N., Soleimany, A. P., Ferris, A. C., Aaronson, S., & Lu, T. K. (2016). Synthetic recombinase-based state machines in living cells. Science, 353(6297), aad8559. doi:10.1126/science.aad8559
Grindley N. D. (1997). Site-specific recombination: synapsis and strand exchange revealed. Current biology : CB, 7(10), R608–R612. https://doi.org/10.1016/s0960-9822(06)00314-9