Coding

Part:BBa_K5035004:Design

Designed by: Lucas Garcia   Group: iGEM24_IEA   (2024-09-28)
Revision as of 03:21, 28 September 2024 by Lucas-gar (Talk | contribs)

A0A419DFF0 is a corrinoid iron-sulfur-containing reductive dehalogenase produced by the Dehalococcoidia bacterium. A0A419DFF0 is structurally similar to enzyme A6RdhA, a corrinoid iron-sulfur-containing reductive dehalogenase capable of degrading PFOA. Because of A0A419DFF0's structural and familial similarity to A6RdhA, it is predicted to have similar PFOA degrading abilities.

A hexahistidine tag for nickel column purification was added to A0A419DFF0.

References:

Momper, Lily et al. “Energy and carbon metabolisms in a deep terrestrial subsurface fluid microbial community.” The ISME journal vol. 11,10 (2017): 2319-2333. doi:10.1038/ismej.2017.94