Coding

Part:BBa_K5035004:Design

Designed by: Lucas Garcia   Group: iGEM24_IEA   (2024-09-28)
Revision as of 03:07, 28 September 2024 by Registry (Talk | contribs)

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A0A419DFF0 reductive dehalogenase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 52
    Illegal PstI site found at 787
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 52
    Illegal PstI site found at 787
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 52
    Illegal BamHI site found at 335
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 52
    Illegal PstI site found at 787
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 52
    Illegal PstI site found at 787
    Illegal NgoMIV site found at 82
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A hexahistidine tag for nickel column purification was added to the A0A419DFF0 sequence.


Source

ATGCATCATCATCATCATCATTCTAAATACCATAGCATTCTGTCCCGCCGCGAATTCCTGAAAGCACTGGGCATGGGTTCTGCCGGCCTGGGTGCACTGGCAGTCGCACCACCTGCGATCCGTGACATGGACGAAGTAATCGCGAGCCCTCAAGCAGACTGGAAACGTCCGGCTTGGGTTAAAGAGGTTGACAAACCGACTGTAGAAATCGACTGGAAAATCCTGGAACGTTTTGATTACCGCGAGGTAATGTTCGTCAACGGTTTCACCAAAGCGGTTGGCAAAGAGACTGCTGGCATCCTGTCTCAGGTGGGCCTGGCGAACGCGAAAAAATGGATCCGCGATAAACGCCCGGGTCACACCCTGAAAGACTACGCTGTTAAGATGGGTGCGCAAGGCTACGCGTTTGACCCGCACTCCTACCTGGGCTACAAAAAATCTCCGACGCCGGAATCTCTGGGTGTGCCACGCTGGGAAGGTACTCCGGAGGAGAACGCGCGCATGGTTCGTACCGTCATGCGTCTGTATGGCGCAGTAGATGTGGCTTATGTTGAACTGGACACCGACACCACTGAAAAACTGATTTACACCTACGATGTCGATGGCAAACAAATGGAAATTAAAGACGTTGAGCTGGGCGAAGAAAGCGAAAAAGTTAAAATCCTGCCGAAGAAAGCCCGCTGGGTGATCGTATACACTATGATGATGTCCCACGAACTGACCCGCCGTCTGCCGTCCTTCAACGCTGAAGCTACCGTATACATGCCGTACGCTCAGGGTCCATGGCTGCAGGATCGTTTTCAGGATTTCATCCGCACCCTGGGTTACAACTGCTACGGTGAACCGCGTCCAAACGCACTGGGTACGTCCGTCGGCCTGGGTGTTATGGGCGGTCTGGGCGAAATCTCCCGCATCGAACACATCATTACGCCGGGTCGTGGCTTTTCTCACCGCGTTTTTAAGATGATCACGGATCTGCCGCTGGCACCGACCAAACCGATCGATACTGGTGTTATGGATTTCTGTCGTGTCTGCAAGAAATGCTCCGACATGTGCCCGGCGCACGCAATTGCGCCGGATACCGAACCGACTTGGAAAATCCCGGGTCCTTATAAAAACCCGGGTGTTAAAGGTTGGTTCCGCATTGAGCCTCTGTGCTATACGTATTGGCGTCAAACGGGTACGGGTTGCGGCTTTTGTCTGGCCCTGTGTCCGCTGAACCGTCCGCAGAACACGTCTTACTTCAAAACGATGCGCTCTACCATCGCTAAAACCACTGCCCTGAACCGCACTTTCCGCAAAATGGATGACCTGCTGGATTGGGGCCCACGCCGTGATCCAGCAGGCTTCTGGGACCTGGAAATGCCATCTTTTGGTTGGGATCTACTCTGGCGTCGATGA

References