DNA

Part:BBa_K5115065

Designed by: Yi Shi   Group: iGEM24_Fudan   (2024-09-20)
Revision as of 18:56, 27 September 2024 by Shiyi2003 (Talk | contribs)


cso, without csoS3

contributed by Fudan iGEM 2023

Introduction

Usage and Biology

Characterization

Agarose gel electrophoresis

contributed by Fudan iGEM 2024
Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.

M: DNA Marker; Lanes 1-8: Amplification of specific regions corresponding to csoS2, csoS3, csoS4A, csoS4B, csoS1C, csoS1A, csoS1B, and csoS1D, demonstrating the presence of the expected subunits derived from the carboxysome plasmid without the csoS3 component.

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 133
    Illegal NotI site found at 5051
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 291
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 799
    Illegal AgeI site found at 1750
    Illegal AgeI site found at 2431
    Illegal AgeI site found at 3385
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 191


References

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Categories
Parameters
None