Composite

Part:BBa_K5398631:Design

Designed by: ziyi guo   Group: iGEM24_NAU-CHINA   (2024-09-27)
Revision as of 17:27, 27 September 2024 by Ian-kwok (Talk | contribs) (→‎References)

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Use RAT terminator sequence to improve the leakage BBa_K3739064 system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 379
    Illegal BamHI site found at 197
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 255
    Illegal AgeI site found at 365
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

The synthetic sequences produced by the company.

References

[1] Premkumar Jayaraman, Kavya Devarajan, Tze Kwang Chua, et al. Blue light-mediated transcriptional activation and repression of gene expression in bacteria[J]. Nucleic Acids Research, 2016, 44(14): 6994-7005.

[2] Renmei Liu, Jing Yang, Jing Yao, et al. Optogenetic control of RNA function and metabolism using engineered light-switchable RNA-binding proteins[J]. Nature Biotechnology, 2022, 40(5): 779-786.